# New AWG Subgroup Announcement: NBISC100 RR-10 Kidney **URL:** \ **Category:** Animal AWG Open Projects\ **Tags:** rna-seq, animalawg, subgroups\ **Created:** [February 19, 2025, 8:11am UTC](https://awg.osdr.space/t/new-awg-subgroup-announcement-nbisc100-rr-10-kidney/1888 "2025-02-19T08:11:51Z")\ **Posts on this page:** 9\ **Page:** 1
**Author:** ![asaravia](https://sea1.discourse-cdn.com/flex001/user_avatar/awg.osdr.space/asaravia/32/290_2.png) [@asaravia](https://awg.osdr.space/u/asaravia)\ **Post date:** [February 19, 2025, 8:11am UTC](https://awg.osdr.space/t/new-awg-subgroup-announcement-nbisc100-rr-10-kidney/1888/1 "2025-02-19T08:11:51Z")
Kaavya and I will be announcing a new sub-group at the next Animal AWG meeting on Wednesday February 26th from 9am - 1030am PT. **NBISC100 RR-10 Kidney AWG Subgroup** Description/Purpose: Compare data generated with the mRNA (UPX and mRNA kits) and ribodepletion methods used in the NBISC100, Kidney RR-10, and similar studies. Ultimately, there are three primary goals of this analysis: - To determine the efficacy of the UPX kit as a potential sequencing method, by comparing its findings with those of the other methods from similar tissues/experiments. - To utilize OSDR resources, such as the multi-study visualization platform to generate manuscript-ready figures. - To evaluate the effect of spaceflight on gene expression in mouse Gastrocnemius, Spleen, Thymys, and Kidney tissues If you are interested in joining this subgroup, please attend the next Animal AWG monthly meeting to learn more. [@AnimalAWG](https://awg.osdr.space/groups/animalawg) ---
**Author:** ![ccnaney](https://sea1.discourse-cdn.com/flex001/user_avatar/awg.osdr.space/ccnaney/32/747_2.png) [@ccnaney](https://awg.osdr.space/u/ccnaney)\ **Post date:** [February 26, 2025, 5:02pm UTC](https://awg.osdr.space/t/new-awg-subgroup-announcement-nbisc100-rr-10-kidney/1888/2 "2025-02-26T17:02:37Z")
Hi, My name is Charley Naney and I’m a Bioscience PhD student and I would like to listen in to the chat today in case there’s an opportunity to learn. You mentioned in notes you were currently looking at effects of Spaceflight on mouse different tissues (liver), and this would be especially helpful for me to learn more about right now. I also realized it’s meeting time now so if you can’t add me until next month I totally understand. Thank you! Best, Charley ---
**Author:** ![asaravia](https://sea1.discourse-cdn.com/flex001/user_avatar/awg.osdr.space/asaravia/32/290_2.png) [@asaravia](https://awg.osdr.space/u/asaravia)\ **Post date:** [March 21, 2025, 4:44pm UTC](https://awg.osdr.space/t/new-awg-subgroup-announcement-nbisc100-rr-10-kidney/1888/3 "2025-03-21T16:44:43Z")
Hi @ccnaney, Thanks for your interest in the subgroup. I’ll be sending out a poll via e-mail to assess people’s availability for a recurring monthly meeting. ---
**Author:** ![asaravia](https://sea1.discourse-cdn.com/flex001/user_avatar/awg.osdr.space/asaravia/32/290_2.png) [@asaravia](https://awg.osdr.space/u/asaravia)\ **Post date:** [April 4, 2025, 5:34am UTC](https://awg.osdr.space/t/new-awg-subgroup-announcement-nbisc100-rr-10-kidney/1888/4 "2025-04-04T05:34:01Z")
The NBISC100 AWG subgroup monthly meeting will take place on the third Thursday of each month, 8-9am PT | 11am-12pm ET | 3-4pm GMT starting on Thursday, May 15th. To receive a calendar invite, add your information to the table on the [NBISC\_Subgroup\_Members](https://docs.google.com/document/d/1DOBTU9YNkaTncPj81OWkUiYcWAtMgJRftLctCXdNjZQ) google doc. [@AnimalAWG](https://awg.osdr.space/groups/animalawg) ---
**Author:** ![asaravia](https://sea1.discourse-cdn.com/flex001/user_avatar/awg.osdr.space/asaravia/32/290_2.png) [@asaravia](https://awg.osdr.space/u/asaravia)\ **Post date:** [May 13, 2025, 4:30pm UTC](https://awg.osdr.space/t/new-awg-subgroup-announcement-nbisc100-rr-10-kidney/1888/5 "2025-05-13T16:30:08Z")
NBISC100 project google drive: > **[NBISC100\_AWG\_Subgroup - Google Drive](https://drive.google.com/drive/folders/1ULGFzvNQXJwjIAH_1L9yUIsVcFx-GINr)** ---
**Author:** ![asaravia](https://sea1.discourse-cdn.com/flex001/user_avatar/awg.osdr.space/asaravia/32/290_2.png) [@asaravia](https://awg.osdr.space/u/asaravia)\ **Post date:** [June 16, 2025, 9:11pm UTC](https://awg.osdr.space/t/new-awg-subgroup-announcement-nbisc100-rr-10-kidney/1888/6 "2025-06-16T21:11:43Z")
Hi NBISC100 AWG subgroup members, I’m excited to announce that the 3 gastrocnemius datasets we’ll be using have been reprocessed with [RNAseq pipeline version G](https://github.com/nasa/GeneLab_Data_Processing/blob/master/RNAseq/Pipeline_GL-DPPD-7101_Versions/GL-DPPD-7101-G.md). Also, since our June meeting falls on a holiday, I have rescheduled the meeting to **Thursday, June 26th at the same time (8am PDT)**. I hope you all can make it (if not, the meeting will be recorded). Please go ahead and checkout the newly processed gastrocnemius datasets ahead of our meeting next Thursday. - [OSD-101](https://osdr.nasa.gov/bio/repo/data/studies/OSD-101) - [OSD-401](https://osdr.nasa.gov/bio/repo/data/studies/OSD-401) - [OSD-419](https://osdr.nasa.gov/bio/repo/data/studies/OSD-419) [@AnimalAWG](https://awg.osdr.space/groups/animalawg) #AWG Discussions #nbisc100 ---
**Author:** ![asaravia](https://sea1.discourse-cdn.com/flex001/user_avatar/awg.osdr.space/asaravia/32/290_2.png) [@asaravia](https://awg.osdr.space/u/asaravia)\ **Post date:** [June 20, 2025, 9:27am UTC](https://awg.osdr.space/t/new-awg-subgroup-announcement-nbisc100-rr-10-kidney/1888/7 "2025-06-20T09:27:01Z")
Since several of you will not be able to make it to the meeting on Friday (6/20), I moved this one more time to next **Thursday, 6/26 at 8am PDT**. I hope more of you will be able to make the new date/time. See you all next week. #nbisc100 ---
**Author:** ![asaravia](https://sea1.discourse-cdn.com/flex001/user_avatar/awg.osdr.space/asaravia/32/290_2.png) [@asaravia](https://awg.osdr.space/u/asaravia)\ **Post date:** [June 26, 2025, 11:51pm UTC](https://awg.osdr.space/t/new-awg-subgroup-announcement-nbisc100-rr-10-kidney/1888/8 "2025-06-26T23:51:24Z")
Hi all, For those of you who couldn’t make it to the meeting today, the recording is uploaded on our google drive here: > **[Meeting\_Recordings - Google Drive](https://drive.google.com/drive/folders/1l-LG_RRndzLImgJfhJdUcoy80VujoJ60)** Notes from today’s meeting are on our notes page here: > **[NBISC100\_AWG\_subgrp\_Meeting\_Notes](https://docs.google.com/document/d/1jHhvOLbsvkRg5KoXwm22Ew1YLmtlX58yF4loLNLobXQ/edit?tab=t.0)** > > NBISC100 AWG Sub-group Meeting Notes Document Leads: Amanda Saravia-Butler and Kaavya Uppala Purpose: To compare data generated with the mRNA (UPX and mRNA kits) and ribodepletion methods used in the NBISC100, Kidney RR-10, and similar studies.... We went over the gastrocnemius datasets during the meeting today. There are several analyses for these datasets that we can get started on. In the notes for today there is a table that lists each task/assessment along with a description and where to put the scripts you create and the output data. **If you are interested in working on any of these analyses, please put your name in the “Person(s) Doing the Analysis” column.** We will go over the results of these analyses during our meeting next month. Let me know if you have any questions about the assessments listed. @kuppala @sigrid.reinsch @paula6 @ila.jasra @louisdarko @nicholas.brereton @Hari-Partha @jgalazka @keith.siew @lovorkad @ccnaney @KariJ @fumimuratani @amaanarif25 @Jovel718 #nbisc100 #animalawg ---
**Author:** ![nicholas.brereton](https://sea1.discourse-cdn.com/flex001/user_avatar/awg.osdr.space/nicholas.brereton/32/25_2.png) [@nicholas.brereton](https://awg.osdr.space/u/nicholas.brereton)\ **Post date:** [July 17, 2025, 4:34pm UTC](https://awg.osdr.space/t/new-awg-subgroup-announcement-nbisc100-rr-10-kidney/1888/9 "2025-07-17T16:34:18Z")
This is what I mentioned re the Qiagen UPX 3’ library kit (for isoform preservation - only a short a short cDNA fragment is synthesised from the polyA). I’ll have a look though as it’s new to me ![3mRNAInfographicV3-scaled-1](https://us1.discourse-cdn.com/flex001/uploads/osdr/original/2X/e/ef07a08a907c3215c775d7c9a44e9d2fa0c144f1.jpeg) Or around 9min + 45:30 min here (funny not to present the limitations): [https://www.youtube.com/watch?v=X9msf1ZYLyE](https://www.youtube.com/watch?v=X9msf1ZYLyE)