Hai, I hope you all are doing well. This is Carlton Ranjith from Sathybama Institute of Science and Technology, a new member of AWG, i am interested in working on mitochondrial genome dynamics and the mitochondrial heteroplasmy effect of spaceflight on Human skeletal muscle tissue chip. I understand that with transcriptomic data understanding, mitochondrial heteroplasmy will be somewhat limited yet just thought about working on it. The workflow will involve 1. Differential expression of genes involved in mitochondrial biogenesis, respiration, and oxidative phosphorylation pathways in spaceflight. 2. Identification of changes in mitochondrial heteroplasmy patterns and alterations in the distribution of mtDNA variants. 3. Correlating the mitochondrial heteroplasmy dynamics, cellular energetics, and oxidative stress responses in response to spaceflight.
I have performed the initial steps like getting the bam file and also variant calling. but I am just concerned about how to proceed with this proposal in collaboration with the team.
Please let me know if this is possible or what has to be done.
Thankyou
With regards
W.A.Carlton Ranjith