Cell Culture Sample Metadata - Feedback Needed!

AWG Discussions
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Calling all cell culture/metadata experts!

We are gathering input from the community here to standardize the information we collect and report about samples deriving from cell culture-based experiments.

Please review our draft sample metadata table and provide your inputs directly in the sheet (starting at row 20).

You can also add questions, comments, or suggestions here to this thread if you would like to facilitate discussion with other members.

If you prefer to provide your inputs privately, feel free to email me at rachel.r.gilbert@nasa.gov.

Thanks for your input!
Rachel

@AnimalAWG @ALSDAawg @MultiOmicsAWG

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Added a bit. Might drop more later. Adding below for additional column suggestions and otherwise:

Additional considerations:

  • A section for Handling, in the vein of animal husbandry (came to mind when reflecting on 3D culture) to address prior handling/processing/relevant stresses, transfections/transductions, ratios for co-culture and deposition/printing patterns
  • Column for initial ratio of cells in chimeric organoid culturing and co-printing/ co-culture development
  • Column for the tissue, surface, and or material cells are deposited on (assuming not standard labware)
  • For 3D culture, either in sample or assay table: it can be helpful to mention the inner diameter/gauge of needle used to deposit cells into gels and similar media. This can give greater confidence of cell count/estimation in some studies.

Paper that touches microneedle geometry and cell viability:
A) Radware Bot Manager Captcha

Papers on organoids (including chimeric organoids), and tumoroids, and printing/using patient/tissue specific ECM (extracellular matrix)

B) 3D bioprinted mammary organoids and tumoroids in human mammary derived ECM hydrogels - PMC

C) A 3D bioprinter platform for mechanistic analysis of tumoroids and chimeric mammary organoids - PMC

D) Combined 3D bioprinting and tissue-specific ECM system reveals the influence of brain matrix on stem cell differentiation - PMC

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Thank you everyone for the feedback so far!

I went ahead and added the radiation columns that we typically would use to collect metadata for radiation studies, since I had forgotten to include those in the first version of the table I posted.

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